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Adipogel-变废为宝,“脂”贵似金

 Fig. Adipogel forms a viscous droplet when isolated on a petri dish. After further processing, it can be used as a natural extracellular matrix to support new tissue growth. (Credit: N. Sharma)

科学研究中有种悖论,最易被你丢弃的可能就是最宝贵的(what you throw away turns out to be most valuable)。如今这真实的发生在了美国莱斯大学(得克萨斯州休斯敦市,Houston, TX)化学与分子生物工程系的助理教授Deepak Nagrath身上。

Nagrath自2009年加盟莱斯大学,之前他在哈佛医学院任助理研究员。他在哈佛期间,专注研究细胞的体外培养。一次实验中,他发现了一种由脂肪细胞分泌的胶冻样粘稠物质,理所当然的被他丢弃,后来证明这种物质就是众多研究者追寻的一种理想的细胞外基质,现被命名为Adipogel胶。

在Adipogel胶发现之前,生物医学研究中最常用的细胞生长基质为Matrigel胶。Matrigel胶来自小鼠的肿瘤细胞,所以被禁止用于人体。Adipogel胶的性能完全可以与Matrigel胶相比拟,而且价格低廉很多,最重要的是来源与“纯天然”且被大多数人认为是“垃圾”的脂肪。

关于Adipogel胶的研究还在继续,一旦Adipogel胶被“成熟化”,可为细胞与组织工程提供近乎完美的载体,应用前景极为乐观。


参考文献:FASEB J. 2010 Mar 16. [Epub ahead of print]

Adipocyte-derived basement membrane extract with biological activity: applications in hepatocyte functional augmentation in vitro.
Sharma NS, Nagrath D, Yarmush ML.

*Center for Engineering in Medicine/Surgical Services, Massachusetts General Hospital, Harvard Medical School, and The Shriners' Hospitals for Children, Boston, Massachusetts, USA;Department of Biomedical Engineering, Rutgers University, Piscataway, New Jersey, USA; andDepartment of Chemical and Biomolecular Engineering, Rice University, Houston, Texas, USA.

Natural and synthetic biomaterials utilized in tissue engineering applications require a dynamic interplay of complex macromolecular compositions of hydrated extracellular matrices (ECMs) and soluble growth factors. The challenges in utilizing synthetic ECMs is the effective control of temporal and spatial complexity of multiple signal presentation, as compared to natural ECMs that possess the inherent properties of biological recognition, including presentation of receptor-binding ligands, susceptibility to cell-triggered proteolytic degradation, and remodeling. We have developed a murine preadipocyte differentiation system for generating a natural basement membrane extract (Adipogel) comprising ECM proteins (collagen IV, laminin, hyaluronan, and fibronectin) and including relevant growth factors (hepatocyte growth factor, vascular endothelial growth factor, and leukemia inhibitory factor). We have shown the effective utilization of the growth factor-enriched extracellular matrix for enhanced albumin synthesis rate of primary hepatocyte cultures for a period of 10 d as compared to collagen sandwich cultures and comparable or higher function as compared to Matrigel cultures. We have also demonstrated comparable cytochrome P450 1A1 activity for the collagen-Adipogel condition to the collagen double-gel and Matrigel culture conditions. A metabolic analysis revealed that utilization of Adipogel in primary hepatocyte cultures increased serine, glycine, threonine, alanine, tyrosine, valine, methionine, lysine, isoleucine, leucine, phenylalanine, taurine, cysteine, and glucose uptake rates to enhance hepatocyte protein synthesis as compared to collagen double-gel cultures. The demonstrated synthesis, isolation, characterization, and application of Adipogel provide immense potential for tissue engineering and regenerative medicine applications.-Sharma, N. S., Nagrath, D., Yarmush, M. L. Adipocyte-derived basement membrane extract with biological activity: applications in hepatocyte functional augmentation in vitro.

PMID: 20233948 [PubMed - as supplied by publisher]

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    摘要
    Adipogel胶的性能完全可以与Matrigel胶相比拟,而且价格低廉很多,最重要的是来源与“纯天然”且被大多数人认为是“垃圾”的脂肪。
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    Adipogel,Matrigel
     
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